牛卵泡ODF1与ODF2转录组发育相关基因筛选及表达差异分析

doi:10.11843/j.issn.0366-6964.2015.11.007

Abstract

Abstract:

This study was performed to screen genes associated with follicular development from ODF1（The largest follicle at onset of deviation）and ODF2(The second largest follicle at onset of deviation)in transcript levels of bovine.The largest and second largest follicle were collected from onset of deviation then separated granulosa cells from which extracted total RNA.High-throughput deep sequencing was carried out by sequencing platform of Illumina.According to the results of assessment analysis in significant enrichment analysis of Gene Ontology function and differential expression in gene screening.Further，screening differential expression gene by ODF1/ODF2 or ODF2/ODF1>2 as the candidate genes.By real-time fluorescent quantitative PCR(qRT-PCR) was performed to detect the gene expression pattern between DF and SF granulose cells of the first follicular wave during estrous cycle in bovine.Eight candidate genes related to follicular development were screened and 7 genes（BEX2，UBN1，SIK1，SPARCL1，LOC784256，LOC789231，LOC785462）from ODF1/ODF2 and one （SAFB2）from ODF2/ODF1，BEX2，UBN1，LOC784256，LOC789231 expressed in DF was extremely significantly higher than that in SF(P<0.01).But the expression levels of SAFB2 was extremely significantly higher in SF than those in DF(P<0.01) and SIK1，SPARCL1 were significantly greater in SF compared with DF(P<0.05).This indifferences trends was consistent between qRT-PCR test results of BEX2，UBN1，LOC784256，LOC789231，SAFB2 and high-throughput sequencing results of RPKM in ODF1 and ODF2.The qRT-PCR test results of SIK1，SPARCL1 and LOC785462 was opposite with the high-throughput sequencing of the gene RPKM in ODF1 and ODF2.

CLC Number:

S823.2

LI Peng-fei，MENG Jin-zhu，XIE Jian-shan，ZHU Zhi-wei，LIU Yan，JIANG Xiao-long，CHEN Jian-wei，YAO Xiao-lei，ZHAO Miao-miao，Lü Li-hua. Screening and Analyse Study of Genes Associated with Follicular Development in Bovine ODF1 and ODF2 Transcript[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 1961-1966.

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Screening and Analyse Study of Genes Associated with Follicular Development in Bovine ODF1 and ODF2 Transcript

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